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The growth inhibitory effect of NAPQI was evaluated by determining the concentration required to inhibit cell growth by 50% (IC 50 ) with the CellTiter Blue assay (Promega, Madison, WI)

GPR146 deficiency reprograms hepatic glucose and amino acid metabolism Beyond reduced hepatic lipid accumulation, GPR146 deficiency led to significantly elevated levels of hexose metabolites within the pentose phosphate pathway (PPP), including hexose monophosphate and erythrose-4-phosphate (Supplementary Fig

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In the large intestine, gut microbiota, including Firmicutes and Proteobacteria, convert choline into trimethylamine (TMA) (8), which is subsequently absorbed and oxidized in the liver by flavin monooxygenase 3 (FMO3) into trimethylamine-N-oxidase (TMAO) (9)